pG3H-PE3max-attR1R2
(Plasmid #213049)

• Purpose: Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites
• Depositing Lab: Bing Yang
• Publication: Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 10.1016/j.xplc.2023.100741.

Backbone

• Vector backbone: pGreen3
• Vector type: Plant Expression ; Note: this plasmid needs helper pVS1-VIR2 for replication in Agrobacterium
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: The final plasmid derived from this Gateway destination vector needs the helper vector pVS1-VIR2 (Addgene Plasmid #134745) for replication in Agrobacterium.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Cas9 nickase
• Alt name: nCas9
• Species: Synthetic; Streptococcus pyogenes
• Insert Size (bp): 4104
• Mutation: H840A, R240K, N413K,
• GenBank ID: NC_002737

Gene/Insert 2

• Gene/Insert name: M-MLV Reverse Transcriptase
• Alt name: M-MLV RT
• Species: Synthetic; Moloney Murine Leukemia Virus
• Insert Size (bp): 2073
• GenBank ID: AAC82568.2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry