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pG3H-PE3max-attR1R2
(Plasmid #213049)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213049 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGreen3
  • Vector type
    Plant Expression ; Note: this plasmid needs helper pVS1-VIR2 for replication in Agrobacterium
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    The final plasmid derived from this Gateway destination vector needs the helper vector pVS1-VIR2 (Addgene Plasmid #134745) for replication in Agrobacterium.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Cas9 nickase
  • Alt name
    nCas9
  • Species
    Synthetic; Streptococcus pyogenes
  • Insert Size (bp)
    4104
  • Mutation
    H840A, R240K, N413K,
  • GenBank ID
    NC_002737

Gene/Insert 2

  • Gene/Insert name
    M-MLV Reverse Transcriptase
  • Alt name
    M-MLV RT
  • Species
    Synthetic; Moloney Murine Leukemia Virus
  • Insert Size (bp)
    2073
  • GenBank ID
    AAC82568.2

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pG3H-PE3max-attR1R2 was a gift from Bing Yang (Addgene plasmid # 213049 ; http://n2t.net/addgene:213049 ; RRID:Addgene_213049)

  • For your References section:

    Modularly assembled multiplex prime editors for simultaneous editing of agronomically important genes in rice. Gupta A, Liu B, Raza S, Chen QJ, Yang B. Plant Commun. 2023 Oct 26:100741. doi: 10.1016/j.xplc.2023.100741. 10.1016/j.xplc.2023.100741 PubMed 37897041
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