pRS712
(Plasmid
#213041)
-
Purpose(Empty Backbone) CRISPR activation based overexpression in Nakaseomyces (Candida) glabrata
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 213041 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCN-MET3
- Backbone size (bp) 12000
-
Vector typeYeast Expression
-
Selectable markersURA3
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pRS712 was a gift from Rebecca Shapiro (Addgene plasmid # 213041 ; http://n2t.net/addgene:213041 ; RRID:Addgene_213041) -
For your References section:
Functional genetic characterization of stress tolerance and biofilm formation in Nakaseomyces (Candida) glabrata via a novel CRISPR activation system. Maroc L, Shaker H, Shapiro RS. mSphere. 2024 Feb 28;9(2):e0076123. doi: 10.1128/msphere.00761-23. Epub 2024 Jan 24. 10.1128/msphere.00761-23 PubMed 38265239