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pαH-S-RRAR/XBB.1.16
(Plasmid #213037)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213037 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pαH
  • Backbone size w/o insert (bp) 4926
  • Total vector size (bp) 8538
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    s -RRAR.XBB.1.16
  • Alt name
    SARS-CoV 2-Omicron.XBB.1.16 with furin side intact
  • Mutation
    Ectodomain (AAs 1-1208); 682-685 furin site RRAR intact; (T19I,Del24/26,A27S,V83A,G142D,Del144,H146Q, E180V, Q183E, V213E,G252V,G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R408S,K417N,N440K, V445P,G446S,F456L,N460K,S477N,K478R,E484A,F486P,F490S,Q498R,N501Y,Y505H,H655Y,N679K,P681H,N764K,D796Y,Q954H,N969K)
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter CMV
  • Tag / Fusion Protein
    • HRV 3C cleavage site (before tags) 8X His tag 2X Strep-Tag II (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A portion of this plasmid was derived from a plasmid made by Jason McLellan;

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pαH-S-RRAR/XBB.1.16 was a gift from Priyamvada Acharya (Addgene plasmid # 213037 ; http://n2t.net/addgene:213037 ; RRID:Addgene_213037)

  • For your References section:

    SARS-CoV-2 Omicron XBB lineage spike structures, conformations, antigenicity, and receptor recognition. Zhang QE, Lindenberger J, Parsons R, Thakur B, Parks R, Park CS, Huang X, Sammour S, Janowska K, Spence TN, Edwards RJ, Martin M, Williams WB, Gobeil S, Montefiori DC, Korber B, Saunders KO, Haynes BF, Haynes BF, Henderson R, Acharya P. bioRxiv [Preprint]. 2024 Mar 12:2024.02.12.580004. doi: 10.1101/2024.02.12.580004. 10.1101/2024.02.12.580004 PubMed 38405707
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