pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro
(Plasmid #213030)

• Purpose: Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2
• Depositing Lab: Martin S. Taylor
• Publication: Structures, Functions, and Adaptations of the Human LINE-1 ORF2 Protein (unpublished)

Backbone

• Vector backbone: pCEP4 Puro
• Backbone manufacturer: Invitrogen (modified)
• Backbone size w/o insert (bp): 9994
• Total vector size (bp): 16226
• Modifications to backbone: Replacement of stock hygromycin with puromycin
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
• Species: H. sapiens (human)
• Insert Size (bp): 6232
• GenBank ID: AF148856.1
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xFlag (ORF2p) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: PmeI (destroyed during cloning)
• 5′ sequencing primer: CMV_Forward
• 3′ sequencing primer: SV40pA-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry