pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41
(Plasmid #213024)

• Purpose: Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion
• Depositing Labs: Martin S. Taylor, Oliver Weichenrieder
• Publication: Structures, Functions, and Adaptations of the Human LINE-1 ORF2 Protein (unpublished)

Backbone

• Vector backbone: pET41
• Backbone size w/o insert (bp): 6465
• Total vector size (bp): 8937
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: LINE-1 ORF2p (238-1061)
• Alt name: ORF2
• Alt name: ORF2 RT
• Alt name: ORFeusHS
• Species: H. sapiens (human)
• Insert Size (bp): 2472
• GenBank ID: AAC51271.1
• Promoter: T7 / Lac
• Tag / Fusion Protein:
  • His6-MBP-3C (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7, MBP_Fwd
• 3′ sequencing primer: T7 Term

Resource Information

• Supplemental Documents:
  • pAMS823 pMT487 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41.gbk
• A portion of this plasmid was derived from a plasmid made by: The construct is based on the synthetic L1 ORFeus-Hs sequence generated in the lab of Jef Boeke (see W. An et al., Mobile DNA 2, 2011 https://doi.org/10.1186/1759-8753-2-2).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry