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Addgene

PB-3xCTCF-TetO
(Plasmid #213023)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213023 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB-empty
  • Backbone size w/o insert (bp) 2888
  • Total vector size (bp) 12722
  • Vector type
    Mouse Targeting ; Piggybase integration (Transposon)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3xCTCF-TetO
  • Alt name
    3 CTCF binding sites + Tet operator array
  • Species
    Synthetic
  • Insert Size (bp)
    8722

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    TetO array derived from: Edith Heard, Insitut Curie (Masui et al. Cell 2011)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-3xCTCF-TetO was a gift from Luca Giorgetti (Addgene plasmid # 213023 ; http://n2t.net/addgene:213023 ; RRID:Addgene_213023)

  • For your References section:

    Cohesin and CTCF control the dynamics of chromosome folding. Mach P, Kos PI, Zhan Y, Cramard J, Gaudin S, Tunnermann J, Marchi E, Eglinger J, Zuin J, Kryzhanovska M, Smallwood S, Gelman L, Roth G, Nora EP, Tiana G, Giorgetti L. Nat Genet. 2022 Dec;54(12):1907-1918. doi: 10.1038/s41588-022-01232-7. Epub 2022 Dec 5. 10.1038/s41588-022-01232-7 PubMed 36471076
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