PB-empty-DSE-TetO-8kb
(Plasmid
#213022)
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PurposepiggyBac donor vector containing an 8-kb TetO operator array for random integration using PBase transposase
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213022 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePB-empty
- Backbone size w/o insert (bp) 2888
- Total vector size (bp) 12144
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Vector typeMouse Targeting ; Piggybase integration (Transposon)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTetO
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Alt nameTet operator array
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SpeciesSynthetic
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byTetO array was obtained from Edith Heard, Institut Curie (Masui et al, Cell 2011)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-empty-DSE-TetO-8kb was a gift from Luca Giorgetti (Addgene plasmid # 213022 ; http://n2t.net/addgene:213022 ; RRID:Addgene_213022) -
For your References section:
Cohesin and CTCF control the dynamics of chromosome folding. Mach P, Kos PI, Zhan Y, Cramard J, Gaudin S, Tunnermann J, Marchi E, Eglinger J, Zuin J, Kryzhanovska M, Smallwood S, Gelman L, Roth G, Nora EP, Tiana G, Giorgetti L. Nat Genet. 2022 Dec;54(12):1907-1918. doi: 10.1038/s41588-022-01232-7. Epub 2022 Dec 5. 10.1038/s41588-022-01232-7 PubMed 36471076