PB-LacI-eGFP
(Plasmid
#213021)
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PurposepiggyBac donor vector containing LacI-eGFP for random integration by PBase
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213021 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePB-empty
- Backbone size w/o insert (bp) 2888
- Total vector size (bp) 8173
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Vector typeMammalian Expression, Mouse Targeting ; Piggybase integration (Transposon)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLacI**-eGFP
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SpeciesSynthetic
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Tag
/ Fusion Protein
- eGFP
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byLacI** was obtained from Angela Taddei, Institut Curie (Dubarry et al., Genes & Dev 25:1365, 2011)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-LacI-eGFP was a gift from Luca Giorgetti (Addgene plasmid # 213021 ; http://n2t.net/addgene:213021 ; RRID:Addgene_213021) -
For your References section:
Cohesin and CTCF control the dynamics of chromosome folding. Mach P, Kos PI, Zhan Y, Cramard J, Gaudin S, Tunnermann J, Marchi E, Eglinger J, Zuin J, Kryzhanovska M, Smallwood S, Gelman L, Roth G, Nora EP, Tiana G, Giorgetti L. Nat Genet. 2022 Dec;54(12):1907-1918. doi: 10.1038/s41588-022-01232-7. Epub 2022 Dec 5. 10.1038/s41588-022-01232-7 PubMed 36471076