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Addgene

PB-LacI-eGFP
(Plasmid #213021)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213021 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    PB-empty
  • Backbone size w/o insert (bp) 2888
  • Total vector size (bp) 8173
  • Vector type
    Mammalian Expression, Mouse Targeting ; Piggybase integration (Transposon)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LacI**-eGFP
  • Species
    Synthetic
  • Tag / Fusion Protein
    • eGFP

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    LacI** was obtained from Angela Taddei, Institut Curie (Dubarry et al., Genes & Dev 25:1365, 2011)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-LacI-eGFP was a gift from Luca Giorgetti (Addgene plasmid # 213021 ; http://n2t.net/addgene:213021 ; RRID:Addgene_213021)
  • For your References section:

    Cohesin and CTCF control the dynamics of chromosome folding. Mach P, Kos PI, Zhan Y, Cramard J, Gaudin S, Tunnermann J, Marchi E, Eglinger J, Zuin J, Kryzhanovska M, Smallwood S, Gelman L, Roth G, Nora EP, Tiana G, Giorgetti L. Nat Genet. 2022 Dec;54(12):1907-1918. doi: 10.1038/s41588-022-01232-7. Epub 2022 Dec 5. 10.1038/s41588-022-01232-7 PubMed 36471076