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Addgene

pAc5.1B-lambdaN-HA
(Plasmid #21302)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21302 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAc5.1B
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5379
  • Vector type
    Drosophila expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Dh5 alpha or XL1-blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lambdaN-HA
  • Insert Size (bp)
    122

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer Ac5 forward primer site
  • 3′ sequencing primer BGH reverse priming site
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1B-lambdaN-HA was a gift from Elisa Izaurralde (Addgene plasmid # 21302 ; http://n2t.net/addgene:21302 ; RRID:Addgene_21302)

  • For your References section:

    mRNA degradation by miRNAs and GW182 requires both CCR4:NOT deadenylase and DCP1:DCP2 decapping complexes. Behm-Ansmant I, Rehwinkel J, Doerks T, Stark A, Bork P, Izaurralde E. Genes Dev. 2006 Jul 15. 20(14):1885-98. 10.1101/gad.1424106 PubMed 16815998
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