pUC-tRNAGln-BbsI-sU6-Kan
(Plasmid
#213016)
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PurposeVector for Cloning of multiple gRNAs driven by distinct promoters (tRNA-Gln and synthetic U6)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213016 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC
- Backbone size w/o insert (bp) 2600
- Total vector size (bp) 3150
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametRNAGln promoter, sU6 promoter, gRNA scaffolds
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gRNA/shRNA sequenceNA
- Promoter tRNAGln promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13 fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC-tRNAGln-BbsI-sU6-Kan was a gift from Stephanie Roessler (Addgene plasmid # 213016 ; http://n2t.net/addgene:213016 ; RRID:Addgene_213016)