Amber-free HIV-1 deltaRT
(Plasmid #213007)

• Purpose: An replication-incompetent amber-free HIV-1 encodes full-length Env BG505 with RT-deleted Q23 as the backbone.
• Depositing Lab: Maolin Lu
• Publication: Ao et al Cell Chem Biol. 2024 Jan 8:S2451-9456(23)00474-9. doi: 10.1016/j.chembiol.2023.12.017.

Backbone

• Vector backbone: pBluescript II KS (pKS)
• Vector type: Unspecified

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: HIV-1 Q23 ΔRT backbone (reverse transcriptase is deleted)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: Unknown
• 3′ sequencing primer: Unknown

Gene/Insert 2

• Gene/Insert name: Env BG505

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: Unknown
• 3′ sequencing primer: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The amber-free HIV-1 Q23 BG505 deltaRT does not contain reverse transcriptase or amber termination codes. All four amber termination codons in the Pol, Vif, Vpu, and Rev genes were modified to ochre codons to warrant their correct translating terminations under amber suppression conditions. The original wild-type amber-abundant HIV-1 Q23 BG505 was a gift from Julie Overbaugh, Fred Hutchinson Cancer Center, Seattle.

The complete deletion of RT may affect capsid maturation.