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Amber-free HIV-1 deltaRT
(Plasmid #213007)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213007 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II KS (pKS)
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    HIV-1 Q23 ΔRT backbone (reverse transcriptase is deleted)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer Unknown
  • 3′ sequencing primer Unknown
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Env BG505

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer Unknown
  • 3′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The amber-free HIV-1 Q23 BG505 deltaRT does not contain reverse transcriptase or amber termination codes. All four amber termination codons in the Pol, Vif, Vpu, and Rev genes were modified to ochre codons to warrant their correct translating terminations under amber suppression conditions. The original wild-type amber-abundant HIV-1 Q23 BG505 was a gift from Julie Overbaugh, Fred Hutchinson Cancer Center, Seattle.

The complete deletion of RT may affect capsid maturation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Amber-free HIV-1 deltaRT was a gift from Maolin Lu (Addgene plasmid # 213007 ; http://n2t.net/addgene:213007 ; RRID:Addgene_213007)

  • For your References section:

    Bioorthogonal click labeling of an amber-free HIV-1 provirus for in-virus single molecule imaging. Ao Y, Grover JR, Gifford L, Han Y, Zhong G, Katte R, Li W, Bhattacharjee R, Zhang B, Sauve S, Qin W, Ghimire D, Haque MA, Arthos J, Moradi M, Mothes W, Lemke EA, Kwong PD, Melikyan GB, Lu M. Cell Chem Biol. 2024 Jan 8:S2451-9456(23)00474-9. doi: 10.1016/j.chembiol.2023.12.017. 10.1016/j.chembiol.2023.12.017 PubMed 38232732
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