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Addgene

pSH83
(Plasmid #212989)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212989 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSH11
  • Backbone size w/o insert (bp) 5429
  • Total vector size (bp) 7297
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    flp-13 promoter
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    2296
  • Entrez Gene
    flp-13 (a.k.a. CELE_F33D4.3)
  • Promoter flp-13

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer TTGGGCTGCAgcatgAAATTGTGCCTCCTGATGCTGAATCCT
  • 3′ sequencing primer CTCTAGAGGCGCGCCGTTCGAATCGGAACGGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloning Method: In-Fusion cloning

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSH83 was a gift from David M. Miller (Addgene plasmid # 212989 ; http://n2t.net/addgene:212989 ; RRID:Addgene_212989)

  • For your References section:

    C. elegans neurons have functional dendritic spines. Cuentas-Condori A, Mulcahy B, He S, Palumbos S, Zhen M, Miller DM 3rd. Elife. 2019 Oct 4;8. pii: 47918. doi: 10.7554/eLife.47918. 10.7554/eLife.47918 PubMed 31584430
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