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Addgene

pT7CFE1_EVD68_3ABCD_C147R
(Plasmid #212980)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212980 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT7CFE1
  • Backbone manufacturer
    Thermo Scientific
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 5866
  • Vector type
    Mammalian Cell Free Protein Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EV-D68 MO/14/18947 3ABCD C147R
  • Species
    EV-D68 MO/14/18947
  • Insert Size (bp)
    2259
  • Mutation
    changed cysteine 147 from 3C to arginine
  • GenBank ID
    KM851225.1
  • Promoter T7
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CACGATGATAATATGGCCTACCCCTACGACG
  • 3′ sequencing primer CAGTCAGATCTCAGTGGTGGTGGTGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7CFE1_EVD68_3ABCD_C147R was a gift from Karla Kirkegaard (Addgene plasmid # 212980 ; http://n2t.net/addgene:212980 ; RRID:Addgene_212980)
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