pLKO.1 shMTH1-2
(Plasmid #21298)

• Depositing Lab: Bob Weinberg
• Publication: Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):169-74.

Backbone

• Vector backbone: pLKO.1 puro
• Backbone manufacturer: Available at Addgene (#8453)
• Backbone size w/o insert (bp): 7000
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: shMTH1-2
• Alt name: MTH1 shRNA
• Alt name: MTH1
• Species: H. sapiens (human)
• Entrez Gene: NUDT1 (a.k.a. MTH1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: LKO.1 5'

Resource Information

• Reference:
  • Addgene's pLKO.1 protocol
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

shRNA #2 directed against MTH1 sequence 5-GAAATTCCACGGGTACTTCAA-3′.