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pT7CFE1_EVA71_VP12A_C110A
(Plasmid #212976)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212976 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT7CFE1
  • Backbone manufacturer
    Thermo Scientific
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 4949
  • Vector type
    Mammalian Cell Free Protein Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Enterovirus A71 4643 VP12A C110A
  • Species
    EV-A71 4643
  • Insert Size (bp)
    1347
  • Mutation
    changed cysteine 110 from 2A to alanine
  • GenBank ID
    MW298156.1
  • Promoter T7
  • Tag / Fusion Protein
    • HA tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CACGATGATAATATGGCCTACCCCTACGACG
  • 3′ sequencing primer CAGTCAGATCTCAGTGGTGGTGGTGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7CFE1_EVA71_VP12A_C110A was a gift from Karla Kirkegaard (Addgene plasmid # 212976 ; http://n2t.net/addgene:212976 ; RRID:Addgene_212976)
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