ISDra2-TnpB-mCherry-U6-ωRNA*
(Plasmid #212967)

• Purpose: Expression vector for expression of ISDra2-TnpB driven by chicken β-actin promoter and mCherry driven by CMV promoter
• Depositing Lab: Chunlong Xu
• Publication: Li et al Nat Commun. 2024 Jan 27;15(1):831. doi: 10.1038/s41467-024-45197-z.

Backbone

• Vector backbone: unknown
• Total vector size (bp): 8090
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: ISDra2-TnpB-T2A-mCherry
• Species: Synthetic; Deinococcus radiodurans
• Promoter: CAG, T7
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • NLS-FLAG (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: ωRNA*
• Species: Synthetic
• Promoter: U6

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pCX2280_ISDra2-TnpB-mCherry-U6-ωRNA.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Xu Lab plasmid pCX2280.

Please visit https://doi.org/10.1101/2023.06.29.547137 for bioRxiv preprint.