pTransposon.CAG.CasRX-P2A-Blast
(Plasmid #212965)

• Purpose: Episomal plasmid encoding CasRx-P2A under the control of the CAG promoter. The construct is flanked by PB and SB repeats for integration into the cell genome.
• Depositing Lab: Roland Rad
• Publication: Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s41592-024-02190-0.

Backbone

• Vector backbone: pA3
• Total vector size (bp): 10329
• Vector type: Mammalian Expression ; transposon
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CasRx
• Alt name: Cas13d Ruminococcus flavefaciens XPD3002
• Species: Ruminococcus flavefaciens XPD3002
• Insert Size (bp): 3456
• Promoter: CAGGS
• Tag / Fusion Protein:
  • P2A-Blasticidin resistance (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry