pBabe puro MTH1
(Plasmid #21295)

• Depositing Lab: Bob Weinberg
• Publication: Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):169-74.

Backbone

• Vector backbone: pBABE-puro
• Backbone manufacturer: Available at Addgene (#1764)
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MTH1
• Species: H. sapiens (human)
• Entrez Gene: NUDT1 (a.k.a. MTH1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBABE 5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Yusaku Nakabeppu provided pcDEB.MTH1
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The pBabe.puro MTH1 overexpression construct was created by amplifying the MTH1 cDNA from pcDEB.MTH1 (which expresses full-length wild type human MTH1) using PCR
primers to add BamHI and EcoRI cloning sites to the 5' and 3'
ends respectively, and ligating the resulting fragment into the appropriately digested retroviral pBABE puro vector.