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pcDNA3.1-AtSLAC1 6D
(Plasmid #212947)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212947 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5383
  • Total vector size (bp) 7054
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Slow anion channel-associated 1
  • Alt name
    SLAC1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1671
  • Mutation
    T62D/S65D/S107D/S124D/S146D/S152D
  • GenBank ID
    BT002640
  • Entrez Gene
    OZS1 (a.k.a. AT1G12480, CARBON DIOXIDE INSENSITIVE 3, CDI3, F5O11.23, F5O11_23, OZONE-SENSITIVE 1, RADICAL-INDUCED CELL DEATH 3, RCD3, SLAC1, SLOW ANION CHANNEL-ASSOCIATED 1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CGC AAA TGG GCG GTA GGC GTG
  • 3′ sequencing primer CTA GAA GGC ACA GTC GAG GC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-AtSLAC1 6D was a gift from Sangho Lee (Addgene plasmid # 212947 ; http://n2t.net/addgene:212947 ; RRID:Addgene_212947)

  • For your References section:

    Cryo-EM structures of the plant anion channel SLAC1 from Arabidopsis thaliana suggest a combined activation model. Lee Y, Jeong HS, Jung S, Hwang J, Le CTH, Jun SH, Du EJ, Kang K, Kim BG, Lim HH, Lee S. Nat Commun. 2023 Nov 14;14(1):7345. doi: 10.1038/s41467-023-43193-3. 10.1038/s41467-023-43193-3 PubMed 37963863
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