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Addgene

pYSD300
(Plasmid #212930)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212930 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYTK083
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AGA1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    AGA1 (a.k.a. YNR044W)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYSD300 was a gift from Paul Young (Addgene plasmid # 212930 ; http://n2t.net/addgene:212930 ; RRID:Addgene_212930)

  • For your References section:

    A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization of Heterologous Protein Secretion and Surface Display in Saccharomyces cerevisiae. O'Riordan NM, Juric V, O'Neill SK, Roche AP, Young PW. ACS Synth Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743. 10.1021/acssynbio.3c00743 PubMed 38483353
Included in kit:
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