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Addgene

pUC19-THG73tRNA
(Plasmid #21292)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21292 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2600
  • Vector type
    T. thermophila

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T. thermophila tRNA Glutamine (CUA)-G73
  • Alt name
    THG73 suppressor tRNA
  • Species
    T. thermophila
  • Insert Size (bp)
    100
  • Mutation
    N/A

Cloning Information

  • Cloning method Restriction Enzyme
  • 3′ cloning site Fok I (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19-THG73tRNA was a gift from Henry Lester (Addgene plasmid # 21292 ; http://n2t.net/addgene:21292 ; RRID:Addgene_21292)

  • For your References section:

    An engineered Tetrahymena tRNAGln for in vivo incorporation of unnatural amino acids into proteins by nonsense suppression. Saks ME, Sampson JR, Nowak MW, Kearney PC, Du F, Abelson JN, Lester HA, Dougherty DA. J Biol Chem. 1996 Sep 20. 271(38):23169-75. 10.1074/jbc.271.38.23169 PubMed 8798511
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