pCYP59
(Plasmid
#212892)
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PurposeExpresses the Priestia megaterium LRD-modifying cytochrome P450 enzyme
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 212892 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCYP
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecytochrome P450
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Alt nameUniprot ID: Q06069
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SpeciesPriestia megaterium
- Promoter PcymRC
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bycodon optimized and synthesized by vendors
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCYP59 was a gift from Christopher Voigt (Addgene plasmid # 212892 ; http://n2t.net/addgene:212892 ; RRID:Addgene_212892) -
For your References section:
Design of a redox-proficient Escherichia coli for screening terpenoids and modifying cytochrome P450s. Lin GM, Voigt CA. Nature Catalysis (2023) 10.1038/s41929-023-01049-5