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Addgene

pCYP22
(Plasmid #212855)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212855 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCYP
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cytochrome P450
  • Alt name
    Uniprot ID: A0A1Z4J4D1
  • Species
    Scytonema sp. HK-05
  • Promoter PcymRC

Cloning Information

  • Cloning method Unknown

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    codon optimized and synthesized by vendors

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCYP22 was a gift from Christopher Voigt (Addgene plasmid # 212855 ; http://n2t.net/addgene:212855 ; RRID:Addgene_212855)

  • For your References section:

    Design of a redox-proficient Escherichia coli for screening terpenoids and modifying cytochrome P450s. Lin GM, Voigt CA. Nature Catalysis. 6, 1016–1029 (2023). https://doi.org/10.1038/s41929-023-01049-5 10.1038/s41929-023-01049-5
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