TRX1 flag C32S
(Plasmid #21284)

• Depositing Lab: Wang Min
• Publication: Liu et al Circ Res. 2002 Jun 28. 90(12):1259-66.

Backbone

• Vector backbone: pcDNA3-FLAG
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRX1
• Species: H. sapiens (human)
• Insert Size (bp): 317
• Mutation: C32S
• Entrez Gene: TXN (a.k.a. TRDX, TRX, TRX1, TXN1, Trx80)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry