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Addgene

pcDNA5-FRT-TO-EGFR*-CUbo(K63R)-His6
(Plasmid #212820)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212820 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5-FRT/TO
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFR*
  • Species
    H. sapiens (human), S. cerevisiae (budding yeast)
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
  • Tag / Fusion Protein
    • CUbo(K63R)-6xHis (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pHU5144; cloning method: site-directed mutagenesis; CUbo(K63R) codon-optimized for mammalian cells

Please note that this plasmid carries a mutation in the sequence encoding the extracellular domain of EGFR (L437I). This does not affect functionality of the construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-FRT-TO-EGFR*-CUbo(K63R)-His6 was a gift from Helle Ulrich (Addgene plasmid # 212820 ; http://n2t.net/addgene:212820 ; RRID:Addgene_212820)

  • For your References section:

    Ubiquiton-An inducible, linkage-specific polyubiquitylation tool. Renz C, Asimaki E, Meister C, Albanese V, Petriukov K, Krapoth NC, Wegmann S, Wollscheid HP, Wong RP, Fulzele A, Chen JX, Leon S, Ulrich HD. Mol Cell. 2024 Jan 18;84(2):386-400.e11. doi: 10.1016/j.molcel.2023.11.016. Epub 2023 Dec 15. 10.1016/j.molcel.2023.11.016 PubMed 38103558
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