pcDNA5-FRT-TO-EGFR*-CUbo(K63R)-GFP
(Plasmid
#212817)
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PurposeConstitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R)-GFP in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 212817 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA5-FRT/TO
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFR*
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SpeciesH. sapiens (human), S. cerevisiae (budding yeast)
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Entrez GeneEGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
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Tag
/ Fusion Protein
- CUbo(K63R)-GFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pHU4742; cloning method: site-directed mutagenesis; CUbo(K63R) codon-optimized for mammalian cells
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5-FRT-TO-EGFR*-CUbo(K63R)-GFP was a gift from Helle Ulrich (Addgene plasmid # 212817 ; http://n2t.net/addgene:212817 ; RRID:Addgene_212817) -
For your References section:
Ubiquiton-An inducible, linkage-specific polyubiquitylation tool. Renz C, Asimaki E, Meister C, Albanese V, Petriukov K, Krapoth NC, Wegmann S, Wollscheid HP, Wong RP, Fulzele A, Chen JX, Leon S, Ulrich HD. Mol Cell. 2024 Jan 18;84(2):386-400.e11. doi: 10.1016/j.molcel.2023.11.016. Epub 2023 Dec 15. 10.1016/j.molcel.2023.11.016 PubMed 38103558