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Addgene

pd2EGFP-1
(Plasmid #21280)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21280 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pd2EGFP1
  • Backbone manufacturer
    Clontech
  • Backbone size (bp) 4300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Clontech discontinued plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

d2EGFP reporter. Used to monitor transcription from cis-regulatory elements. You must insert a promoter to get d2EGFP expression (d2EGFP has a faster turnover rate than EGFP, making it more accurate for promoter experiments.)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pd2EGFP-1 was a gift from Frank Costantini (Addgene plasmid # 21280)

  • For your References section:

    Wnt/beta-catenin/Tcf signaling induces the transcription of Axin2, a negative regulator of the signaling pathway. Jho EH, Zhang T, Domon C, Joo CK, Freund JN, Costantini F. Mol Cell Biol. 2002 Feb . 22(4):1172-83. 10.1128/MCB.22.4.1172-1183.2002 PubMed 11809808
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