Axin2-d2EGFP
(Plasmid #21276)

• Depositing Lab: Frank Costantini
• Publication: Jho et al Mol Cell Biol. 2002 Feb . 22(4):1172-83.

Backbone

• Vector backbone: d2EGFP-1
• Backbone manufacturer: Clontech (discontinued)
• Backbone size w/o insert (bp): 4300
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Axin2 promoter
• Alt name: Axin2
• Species: M. musculus (mouse)
• Insert Size (bp): 5600
• Entrez Gene: Axin2 (a.k.a. A, Axi1, Axil, Condu, Conductin)
• Tag / Fusion Protein:
  • d2EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: HindIII / SmaI (destroyed during cloning)
• 5′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The 5.6-kb DNA fragment was excised from Axin2-Luciferase using KpnI and HindIII (the HindIII site was filled in with Klenow DNA polymerase) and cloned into the KpnI- and SmaI-digested pd2EGFP-1 vector.