pET24a-NUbo-MCS-His6
(Plasmid
#212723)
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Purpose(Empty Backbone) Bacterial expression of a protein of interest with N-terminal NUbo- and C-terminal His6-fusions, contains multiple cloning site
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 212723 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET24a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pHU5793
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET24a-NUbo-MCS-His6 was a gift from Helle Ulrich (Addgene plasmid # 212723 ; http://n2t.net/addgene:212723 ; RRID:Addgene_212723) -
For your References section:
Ubiquiton-An inducible, linkage-specific polyubiquitylation tool. Renz C, Asimaki E, Meister C, Albanese V, Petriukov K, Krapoth NC, Wegmann S, Wollscheid HP, Wong RP, Fulzele A, Chen JX, Leon S, Ulrich HD. Mol Cell. 2024 Jan 18;84(2):386-400.e11. doi: 10.1016/j.molcel.2023.11.016. Epub 2023 Dec 15. 10.1016/j.molcel.2023.11.016 PubMed 38103558