Skip to main content
Addgene

pET15b-His6-CUbo-GFP
(Plasmid #212720)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212720 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • Species
    H. sapiens (human), S. cerevisiae (budding yeast)
  • Tag / Fusion Protein
    • 6xHis-CUbo (N terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pHU3900; cloning method: site-directed mutagenesis

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b-His6-CUbo-GFP was a gift from Helle Ulrich (Addgene plasmid # 212720 ; http://n2t.net/addgene:212720 ; RRID:Addgene_212720)

  • For your References section:

    Ubiquiton-An inducible, linkage-specific polyubiquitylation tool. Renz C, Asimaki E, Meister C, Albanese V, Petriukov K, Krapoth NC, Wegmann S, Wollscheid HP, Wong RP, Fulzele A, Chen JX, Leon S, Ulrich HD. Mol Cell. 2024 Jan 18;84(2):386-400.e11. doi: 10.1016/j.molcel.2023.11.016. Epub 2023 Dec 15. 10.1016/j.molcel.2023.11.016 PubMed 38103558
Included in kit:
Related items: