pcDNA3.1_hSMAD4
(Plasmid #212710)

• Purpose: The ORF of human SMAD4, variant 1 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.
• Depositing Labs: Catharine Ross, Reza Zolfaghari
• Publication: Zolfaghari and Ross plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 7087
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Homo sapiens SMAD4
• Species: H. sapiens (human)
• Insert Size (bp): 1659
• GenBank ID: NM_005359
• Entrez Gene: SMAD4 (a.k.a. DPC4, JIP, MADH4, MYHRS)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: TTG CTA GCG CCA CCA TGG ACA ATA TGT CTA TTA C
• 3′ sequencing primer: TTA AGC TTT CAG TCT AAA GGT TGT GGG TC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry