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Addgene

pCfB8860
(Plasmid #212697)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212697 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    custom backbone
  • Vector type
    Yeast Expression
  • Selectable markers
    Hph

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA targeting to the intergradtion site E3
  • gRNA/shRNA sequence
    Unknown
  • Species
    Other

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCfB8860 was a gift from Irina Borodina (Addgene plasmid # 212697 ; http://n2t.net/addgene:212697 ; RRID:Addgene_212697)

  • For your References section:

    Combinatorial iterative method for metabolic engineering of Yarrowia lipolytica: application for betanin biosynthesis. Jiang W, Wang S, Avila P, Jorgensen TS, Yang Z, Borodina I. Metab Eng. 2024 Sep 9:S1096-7176(24)00110-1. doi: 10.1016/j.ymben.2024.09.003. 10.1016/j.ymben.2024.09.003 PubMed 39260817
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