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pCW-HA-hRb-delta-CDK-T373D-S608D-S612D-puro
(Plasmid #212675)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212675 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCW
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rb
  • Species
    H. sapiens (human)
  • Mutation
    15 CDK phospho-sites are mutated to alanines, and T373D/S608D/S612D
  • Entrez Gene
    RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110)
  • Promoter TRE
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW-HA-hRb-delta-CDK-T373D-S608D-S612D-puro was a gift from Yumi Konagaya & Tobias Meyer (Addgene plasmid # 212675 ; http://n2t.net/addgene:212675 ; RRID:Addgene_212675)

  • For your References section:

    An intermediate Rb-E2F activity state safeguards proliferation commitment. Konagaya Y, Rosenthal D, Ratnayeke N, Fan Y, Meyer T. Nature. 2024 Jul;631(8020):424-431. doi: 10.1038/s41586-024-07554-2. Epub 2024 Jun 26. 10.1038/s41586-024-07554-2 PubMed 38926571
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