pET26-Ufd1-His
(Plasmid #21266)

• Depositing Lab: Hemmo Meyer
• Publication: Bruderer et al J Biol Chem. 2004 Nov 26. 279(48):49609-16.

Backbone

• Vector backbone: pET26
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Ufd1
• Alt name: UFD1L
• Species: M. musculus (mouse)
• Insert Size (bp): 921
• Entrez Gene: Ufd1l (a.k.a. Ufd1)
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene Next-generation sequencing QC processes identified that the insert harbors truncations of the region encoding the first 4 amino acids and final amino acid of Ufd1, relative to the NCBI reference sequence NM_011672