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Addgene

pLV_CAG_Gag∆MA
(Plasmid #212633)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212633 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeocin ; GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MLV Gag (UniProt ID: P03332)
  • Species
    MoMLV
  • Mutation
    Deletion of MA domain (amino acids 2-215)
  • Entrez Gene
    gag (a.k.a. MLVgp8)
  • Promoter CAG

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Amino acids 216-530 of the MLV Gag ORF were cloned from MLV Gag-YFP (Addgene Plasmid #1813, a gift from Walther Mothes)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV_CAG_Gag∆MA was a gift from Paul Blainey (Addgene plasmid # 212633 ; http://n2t.net/addgene:212633 ; RRID:Addgene_212633)

  • For your References section:

    Live-cell transcriptomics with engineered virus-like particles. Najia, M. A., Borrajo, J., Le, A., Tsai, F., Huang, J. Y., Griffith, L. G., Daley, G. Q., & Blainey, P. C.. bioRxiv. 10.1101/2024.10.01.616098
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