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Addgene

CROPseq-Puro-4xMS2
(Plasmid #212626)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212626 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CROPseq-Guide-Puro (Addgene #86708)
  • Backbone manufacturer
    Christoph Bock Lab
  • Total vector size (bp) 10770
  • Modifications to backbone
    MS2 hairpins were introduced into the 3' UTR of the puromycin mRNA transcript, and the sgRNA scaffold sequence was derived from Dang, et al. (2015). Genome Biology (PMID: 26671237)
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
  • gRNA/shRNA sequence
    replace stuffer sequence with custom sgRNA spacer sequence via BsmBI Golden Gate cloning
  • Species
    Synthetic
  • Promoter EF1a

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CROPseq-Puro-4xMS2 was a gift from Paul Blainey (Addgene plasmid # 212626 ; http://n2t.net/addgene:212626 ; RRID:Addgene_212626)

  • For your References section:

    Live-cell transcriptomics with engineered virus-like particles. Najia, M. A., Borrajo, J., Le, A., Tsai, F., Huang, J. Y., Griffith, L. G., Daley, G. Q., & Blainey, P. C.. bioRxiv. 10.1101/2024.10.01.616098
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