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Addgene

CFP-C1-PLCdelta-PH
(Plasmid #21262)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21262 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4701
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PLCdelta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    528
  • Mutation
    PH domain of PLCdelta (human). Also known as PLCdelta-PH-CFP. Lab plasmid WC0026.
  • Entrez Gene
    PLCD1 (a.k.a. NDNC3, PLC-III)
  • Tag / Fusion Protein
    • CFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CFP-C1-PLCdelta-PH was a gift from Tobias Meyer (Addgene plasmid # 21262 ; http://n2t.net/addgene:21262 ; RRID:Addgene_21262)

  • For your References section:

    Localized biphasic changes in phosphatidylinositol-4,5-bisphosphate at sites of phagocytosis. Botelho RJ, Teruel M, Dierckman R, Anderson R, Wells A, York JD, Meyer T, Grinstein S. J Cell Biol. 2000 Dec 25. 151(7):1353-68. 10.1083/jcb.151.7.1353 PubMed 11134066
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