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Addgene

Noodles Plasmid
(Plasmid #212613)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212613 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmirGLO Dual Luciferase miRNA Target Expression Vector
  • Backbone manufacturer
    Promega (E1330)
  • Backbone size (bp) 7357
  • Modifications to backbone
    Split Luciferase Cassette insertion Cas9-sgRNA cassette insertion
  • Vector type
    Mammalian Expression, CRISPR, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene Plasmid # 132962, Addgene Plasmid # 98290

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Noodles Plasmid was a gift from Ilya Vinnikov (Addgene plasmid # 212613 ; http://n2t.net/addgene:212613 ; RRID:Addgene_212613)

  • For your References section:

    Noodles, the all-in-one system for on-target efficiency analysis of CRISPR guide RNAs. Lin D, Najam SS, Liu Y, Murgia N, Vinnikov IA. MethodsX. 2023 Nov 15;12:102481. doi: 10.1016/j.mex.2023.102481. eCollection 2024 Jun. 10.1016/j.mex.2023.102481 PubMed 38162150
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