pcDNA3.1+-Tornado-split-nLuc
(Plasmid
#212612)
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PurposeFor the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 212612 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1+
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 7125
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTornado-CVB3-split-nLuc
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Insert Size (bp)2673
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MutationC373T mutation in CVB3
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site BsiWI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1+-Tornado-split-nLuc was a gift from Samie Jaffrey (Addgene plasmid # 212612 ; http://n2t.net/addgene:212612 ; RRID:Addgene_212612) -
For your References section:
Highly efficient cellular expression of circular mRNA enables prolonged protein expression. Unti MJ, Jaffrey SR. Cell Chem Biol. 2023 Oct 13:S2451-9456(23)00334-3. doi: 10.1016/j.chembiol.2023.09.015. 10.1016/j.chembiol.2023.09.015 PubMed 37883972
