pcDNA3.1+-Tornado-split-nLuc
(Plasmid #212612)

• Purpose: For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.
• Depositing Lab: Samie Jaffrey
• Publication: Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(23)00334-3. doi: 10.1016/j.chembiol.2023.09.015.

Backbone

• Vector backbone: pcDNA3.1+
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 7125
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tornado-CVB3-split-nLuc
• Insert Size (bp): 2673
• Mutation: C373T mutation in CVB3
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: BsiWI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry