Krox20 promoter pGL3-TATA
(Plasmid #21260)

• Depositing Lab: Jerry Crabtree
• Publication: Kao et al Science. 2009 Jan 30. 323(5914):651-4.

Backbone

• Vector backbone: pGL3
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4818
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Krox20 promoter
• Alt name: Krox20
• Species: M. musculus (mouse)
• Insert Size (bp): 1341
• Entrez Gene: Egr2 (a.k.a. Egr-2, Krox-20, Krox20, NGF1-B, Zfp-25, Zfp-6)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn1 (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a T to C mismatch with Krox20. The single mis-match of the nucleotide is likely to reflect the polymorphism of different mouse strain used in the cloning.