HOXB6-pCMV
(Plasmid
#21258)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21258 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV-Tag2
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4300
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Vector typeMammalian Expression, Bacterial Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHOXB6
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SpeciesH. sapiens (human)
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Insert Size (bp)1000
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Entrez GeneHOXB6 (a.k.a. HOX2, HOX2B, HU-2, Hox-2.2)
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Tag
/ Fusion Protein
- FLAG_Pro Tag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HOXB6-pCMV was a gift from Corey Largman (Addgene plasmid # 21258 ; http://n2t.net/addgene:21258 ; RRID:Addgene_21258) -
For your References section:
The HOX homeodomain proteins block CBP histone acetyltransferase activity. Shen WF, Krishnan K, Lawrence HJ, Largman C. Mol Cell Biol. 2001 Nov;21(21):7509-22 10.1128/MCB.21.21.7509-7522.2001 PubMed 11585930
Map uploaded by the depositor.
