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Addgene

pSCALPS-Flag-Miwi-EGFP
(Plasmid #212575)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212575 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSCALPS
  • Backbone size w/o insert (bp) 7743
  • Total vector size (bp) 12295
  • Modifications to backbone
    EGFP added in frame of insert
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Miwi
  • Alt name
    Piwil1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3299
  • GenBank ID
    NM_021311
  • Entrez Gene
    Piwil1 (a.k.a. MIWI)
  • Promoter SFFV
  • Tags / Fusion Proteins
    • 5x Flag (N terminal on insert)
    • SNAP tag (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GAGGCCAAGAACAGATGGTCC
  • 3′ sequencing primer CAACGAGAAGCGCGATCACATG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSCALPS-Flag-Miwi-EGFP was a gift from Phillip Zamore (Addgene plasmid # 212575 ; http://n2t.net/addgene:212575 ; RRID:Addgene_212575)

  • For your References section:

    GTSF1 accelerates target RNA cleavage by PIWI-clade Argonaute proteins. Arif A, Bailey S, Izumi N, Anzelon TA, Ozata DM, Andersson C, Gainetdinov I, MacRae IJ, Tomari Y, Zamore PD. Nature. 2022 Aug;608(7923):618-625. doi: 10.1038/s41586-022-05009-0. Epub 2022 Jun 30. 10.1038/s41586-022-05009-0 PubMed 35772669
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