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pTwist-SARS-CoV-2 Spike-deltaC18 BA.2.86
(Plasmid #212538)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212538 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTwist-CMV-BetaGlobin-WPRE-Neo
  • Backbone manufacturer
    Twist
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 Spike BA.2.86
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Mutation
    See Depositor Comments.
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter CAG

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Relevant mutations: insertion of MPLF after V16 , T19I , R21T , deltaL24P25P26 , A27S , S50L , del H69V70 , V127F , G142D , del Y145 , F157S , R158G , N211I , del L212 , V213G , L216F , H245N , A264D , I332V , G339H , K356T , S371F , S373P , S375F , T376A , R403K , D405N , R405S , K417N , N440K , V445H , G446S , N450D , L452W , N460K , S477N , T478K , N481K , V483K , del E484 , F486P , Q498R N501Y Y505H E554K A570V D614G P621S H655Y N679K P681R N764K D796Y S939F Q954H N969K P1143L Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTwist-SARS-CoV-2 Spike-deltaC18 BA.2.86 was a gift from Alejandro Balazs (Addgene plasmid # 212538 ; http://n2t.net/addgene:212538 ; RRID:Addgene_212538)

  • For your References section:

    Ongoing evolution of SARS-CoV-2 drives escape from mRNA vaccine-induced humoral immunity. Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ, Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran WF, Balazs AB. medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 10.1101/2024.03.05.24303815. 10.1101/2024.03.05.24303815 PubMed 38496628
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