PKC eta CAT
(Plasmid #21247)

• Depositing Lab: Bernard Weinstein
• Publication: Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-16.

Backbone

• Vector backbone: pHACE
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PKC eta
• Species: M. musculus (mouse)
• Insert Size (bp): 1026
• Mutation: delta 1-341
• GenBank ID: J05703 D90242
• Entrez Gene: Prkch (a.k.a. Pkch)
• Tag / Fusion Protein:
  • HA (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: PKC eta was received from Dr. S. Ohno. If you publish a paper with this plasmid, please cite this paper (see the comments section below) as a reference.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Osada, S., Mizuno, K., Saido, T.C., Akita, Y., Suzuki, K., Kuroki, T., and Ohno, S. 1990. A phorbol ester receptor/protein kinase, nPKC eta, a new member of the protein kinase C family predominantly expressed in lung and skin. The Journal of biological chemistry 265(36): 22434-22440.

Please note that Addgene was not able to completely confirm the truncation with quality control sequencing.