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pJet-CMV-IARS1[W435C]-GFP
(Plasmid #212323)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212323 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJET1.2
  • Backbone size w/o insert (bp) 3945
  • Total vector size (bp) 8499
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    IARS
  • Alt name
    IleRS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4554
  • Mutation
    Trp435cys
  • GenBank ID
    NM_002161.5
  • Entrez Gene
    IARS1 (a.k.a. GRIDHH, IARS, ILERS, ILRS, IRS, PRO0785)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJet-CMV-IARS1[W435C]-GFP was a gift from Sabine Fuchs (Addgene plasmid # 212323 ; http://n2t.net/addgene:212323 ; RRID:Addgene_212323)

  • For your References section:

    Isoleucine-to-valine substitutions support cellular physiology during isoleucine deprivation. Kok G, Schene I, Ilcken E, Sobrevals P, Mendes M, Smith D, Salomons G, Shehata S, Jans J, Maroofian R, Hoek T, van Es R, Rehmann H, Nieuwenhuis E, Vos H, Fuchs S. Nucleic Acids Research 10.1093/nar/gkae1184
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