aMHC-mCherry-Rex-Blasticidin
(Plasmid #21228)

• Depositing Lab: Mark Mercola
• Publication: Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Backbone

• Vector backbone: SMPU
• Backbone size w/o insert (bp): 7338
• Vector type: Lentiviral
• Selectable markers: Blasticidin resistanct marker driven by Rex-1 promoter

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: alpha myosin heavy chain promoter
• Alt name: aMHC promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 5470
• Tag / Fusion Protein:
  • mCherry (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: CTT GAA GCG CAT GAA CTC - 3' Sequencing only

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is an extra C in the promoter sequence when compared to the available reference sequence in NCBI. The mismatch is due to older sequencing artifact of an erroneous insertion of a "C" adjacent to "GGG". The construct should be correct.