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Addgene

T/Brachyury-eGFP Rex Neo Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: BglII and EcoRV+NotI

We expected the following band sizes for this digest: 5.2kb + 3.6kb for BglII and 6.6kb + 2.4kb for EcoRV/NotI. We see the expected pattern.