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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21220 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneC1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCAMKIIalpha-T286A
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Alt nameCAMKIIalpha
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SpeciesR. norvegicus (rat)
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MutationT286A (Lab plasmid KS 0003).
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Entrez GeneCamk2a (a.k.a. PK2CDD, PKCCD)
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Tag
/ Fusion Protein
- GFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-C1-CAMKIIalpha-T286A was a gift from Tobias Meyer (Addgene plasmid # 21220 ; http://n2t.net/addgene:21220 ; RRID:Addgene_21220) -
For your References section:
Dynamic control of CaMKII translocation and localization in hippocampal neurons by NMDA receptor stimulation. Shen K, Meyer T. Science. 1999 Apr 2. 284(5411):162-6. 10.1126/science.284.5411.162 PubMed 10102820