pLSU1/Os-ubi:nnLuz-I{PIV-216}:NosT
(Plasmid #212191)

• Purpose: This binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the rice ubiquitin (R. ubi) promoter and UTR
• Depositing Lab: Wayne Curtis
• Publication: Curtis lab plasmids (unpublished)

Backbone

• Vector backbone: pLSU1
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: nnLuz-I{PIV-216}
• Species: Neonothopanus nambi
• Insert Size (bp): 999
• Mutation: PIV intron added at bp 216, eliminates expression in Agrobacterium
• Promoter: Rice ubiquitin

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pLSU1_Os-ubi_nnLuz-I{PIV-216}_NosT.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry