GFP-C1-AKT-PH
(Plasmid #21218)

• Depositing Lab: Tobias Meyer
• Publication: Raucher et al Cell. 2000 Jan 21. 100(2):221-8.

Backbone

• Vector backbone: C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AKT-PH
• Alt name: AKT
• Species: M. musculus (mouse)
• Insert Size (bp): 457
• Mutation: PH domain of AKT. (Lab plasmid WC 0002).
• Tag / Fusion Protein:
  • GFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry